Protein molecule is a biopolymer which is composed of 20 kinds of amino
acids Each amino acid links with the other one to form a molecule
with high molecular weight. Amino acid has a specific side chain; therefore
amino acid sequence and steric form of the protein varies from protein
to protein. Accordingly, each protein function is different from each other.
We have been studying the importance of the protein structure for functioning
specifically in the living organisms. We are also going to find out the
mechanisms how each protein works properly. Actually, we are orking on
two proteins: restriction enzyme HindIII which digests DNA at the specific
nucleotide sequence and intermediate filament, a fiber protein which supports
the cell internally against external force. For these purposes, we clone
the desired gene, determine the nucleotide sequence and express the protein
in Escherichia coli in order to analyze its strucutre and biochemical properties.
Furhter we are preparing mutant forms of the desired protein and compare
them with the wild one.
Research